• 专利附录
  • 专利说明
  • 权利要求
  • 类似技术
  • 同族专利
  • 引用文献
  • 法律状态
  • 优先权
    • 专利摘要:
    The invention relates to a method and system for increasing productivity of hydrogenotrophic microorganisms. The method comprising the steps of: - providing a reactor comprising a reactor vessel, at least one gas inlet and / or hydrogen production system, at least one fluid inlet, and at least one fluid inlet; - providing the reactor with a hydrogenotrophic micro-organism culture; - providing a gas supply to the at least one gas inlet, the gas supply comprising an amount of hydrogen and / or producing hydrogen with the hydrogen production system; - providing a fluid supply to the at least one fluid inlet; - the growth of biomass; and - removing and / or taking up hydrogen (H2), carbon dioxide (CO2) and / or NH3 / NH4 +. The method preferably further comprises the steps of: - providing the reactor with a phototrophic microorganism culture; and - the in-situ formation of oxygen (O2) by the phototrophic microorganisms.
    公开号:BE1025911B1
    申请号:E20185708
    申请日:2018-10-16
    公开日:2019-12-24
    发明作者:Antonie Martinus Verschoor；Tomas Hubertus Johannes Antonius Sleutels；Philipp Kuntke；Hubertus Victor Marie Hamelers；Cees Jan Nico Buisman
    申请人:Avecom Nv；
    IPC主号:
    专利说明:

    METHOD AND SYSTEM FOR INCREASING THE PRODUCTIVITY OF HYDROGENOTROPHIC MICRO-ORGANISMS
    The invention relates to a method for increasing (biomass) productivity of hydrogenotrophic microorganisms. More in particular, the method uses hydrogenotrophic microorganisms, such as bacteria and / or algae, to produce biomass.
    In general, biomass is produced under the influence of light. For example, algae are manufactured and used commercially in food products and feed.
    The growth rate of the biomass is limited by the availability of the gases that participate in the reactions to grow the biomass. This limited availability of the required gas components is caused by the relatively low solubility of these relevant gases and thereby the relatively low gas-to-liquid transfer rate. In addition, valuable hydrogen gas in use is often lost via the so-called bioreactor “off-gas”. If air is used as an oxygen source, the gas must be ventilated to prevent gas accumulation in the process. This reduces the efficiency of growing the biomass. There is also a safety risk when a mixture of hydrogen (H 2 ) and oxygen (O 2 ) is used. This provides for limitations of the process that thereby hinders optimal biomass growth.
    An object of the present invention is to provide a method for increasing the biomass productivity and / or concentration that eliminates or at least reduces the aforementioned problems and / or is more efficient compared to conventional biomass manufacturing methods.
    The object is achieved with the method for increasing the biomass productivity using hydrogenotrophic microorganisms according to the invention, the method comprising the steps of:
    - providing a reactor comprising a reactor vessel, at least one gas inlet and / or hydrogen production system, at least one liquid inlet, and at least one liquid outlet;
    - providing the reactor with a hydrogenotrophic micro-organism culture;
    - providing a gas supply to the at least one gas inlet, the gas supply comprising an amount of hydrogen and / or producing hydrogen with the hydrogen production system;
    - providing a liquid supply to the at least one liquid inlet;
    - the growth of biomass; and
    BE2018 / 5708
    - removing and / or taking up hydrogen (H 2 ), carbon dioxide (CO 2 ) and / or NH 3 / NH 4 +.
    The reactor comprises one or more gas inlets, liquid inlets, liquid outlets, and preferably also gas outlets. This makes it possible to provide a gas supply to the at least one gas inlet, the gas supply comprising an amount of hydrogen gas, and providing a liquid supply and allowing liquid outflow. Additionally, or as an alternative to the gas inlet, a hydrogen production system can be used to produce the required hydrogen in situ, for example with an electrode system mounted in the reactor. A gas outlet is preferably provided to prevent gas from accumulating in the reactor. The hydrogenotrophic microorganisms use the supplied hydrogen to increase the biomass growth using light through the direct and / or indirect formation of oxygen.
    In one of the preferred embodiments, the method further comprises the steps of providing the reactor with at least one phototrophic microorganism culture and in-situ formation of oxygen (O 2 ) by the phototrophic microorganisms.
    Phototrophic microorganisms not only use the hydrogen itself, they can also produce oxygen that can be used by hydrogen oxidizing microorganisms. This has the advantage that the oxidizing products (equivalents) are produced in the reactor itself. More specifically, in situ formed oxygen is obtained by using the phototrophic microorganisms. Therefore, this method eliminates the limitation of the gas-to-liquid transfer of oxygen. Therefore, the method improves the efficiency of the method for increasing the biomass productivity.
    Additionally, in some cases, biomass manufacture is limited by light. In the case of supplying gas under light limiting conditions, the light limitation can be reduced and the productivity or concentration can be increased.
    Furthermore, the generation of oxygen in situ prevents the dilution of hydrogen and / or oxygen by air or oxygen additions. This prevents dilution of hydrogen, thereby allowing higher hydrogen partial pressure, which reduces the gas-to-liquid transition limitation for hydrogen. Furthermore, in the case that air is used, nitrogen gas will be introduced into the system and will typically accumulate in the main room of the reactor. Such an accumulation is prevented by the in-situ formation of oxygen. This further increases biomass productivity.
    In an embodiment of the invention, energy from light is used to cleave water (H 2 O) and assimilate carbon dioxide (CO 2), resulting in the formation of carbohydrates ([CH 2 O]) and oxygen (O 2 ):
    a) H 2 O + CO 2 [CH 2 O] + O 2
    BE2018 / 5708
    Every (phototrophic micro) organism provided with hydrogen is able to oxidize hydrogen in an environment where hydrogen and oxygen are both present, via the so-called oxyhydrogen (Bang gas) reaction:
    b) 2H 2 + O 2 2H 2 O
    This reaction does not require an additional energy source, because the energy released during the oxidation of the hydrogen is sufficient. As a result, the energy can also be used for CO 2 reduction (fixation), for example:
    c) 6H 2 + O 2 + 2 CO 2 2 [CH 2 O] + 4H 2 O
    The aforementioned oxyhydrogen pathway is interesting and can be done in the dark using an inorganic carbon source.
    Phototrophic microorganisms are capable of producing (in-situ) oxygen in the system. By closely monitoring the chemical balance in the system (eg oxygen concentration, redox potential and / or pH), the light energy (and / or: the CO 2 supply) can be adjusted so that there is just enough phototrophic oxygen production in the system ( reaction a) is to react with hydrogen (reaction b / c). Although the net stoichiometry will be comparable to photo reduction,
    d) 2H2 + CO2 H 2 O + [CH 2 O], the interesting aspect of this approach is that it can happen under the high light intensities (ie, high reaction rates) and that the O 2- producing phototrophic organisms can be different from of the hydrogen oxidizers, and even physically separated from each other in the reactor. In principle, the use of a different co-culture, of (photoautotrophic) microorganisms, such as microalgae, and (chemoautotrophic) hydrogen-oxidizing microorganisms, such as bacteria, is possible according to the present invention. This regulatory principle can be applied to any process that requires aerobic / anaerobic conditions and / or partially redox state or gradient, such as Sophie's oxidation reactors (Sophie's oxidation to elemental sulfur: CO 2 + 2H 2 S [CH 2 O ] + H 2 O + 2S [avoidance of total oxidation to sulphate]);
    anammox (ANaerobic AMMonium OXidation) reactors (partly nitrification [nitration]):
    e) NH 4 + + 2 O 2 NO 2 - + 2 H 2 O
    f) NH 4 + + NO 2 - N 2 + 2 H 2 O;
    aerobic granulate reactors, e.g. Nereda or reactors adapted for degradation of organic (micro) contaminants over a redox gradient; and bio-electrochemical systems (i.e., microbial fuel cells, microbial synthesis cells, etc.).
    Therefore, the method according to the invention reduces the growth limitation used for the gas-to-liquid transfer rates, preferably dilution and loss of valuable gases is prevented, and provides for a safer process by forming oxygen in situ.
    BE2018 / 5708
    The benefits are further improved when the method is performed in a closed system in one of the embodiments of the invention. This allows further control of the process steps.
    In a preferred embodiment of the invention, the method further comprises the step of monitoring and / or controlling the in-situ oxygen formed by the phototrophic microorganisms with an in-situ oxygen controller and / or redox controller.
    The in-situ oxygen controller can make controlling the in-situ manufacture of oxygen effective. In preferred embodiments, this controller regulates the in-situ oxygen concentration by regulating one or more of: light intensity, CO2 supply, pH, redox potential. This allows direct in-situ oxygen production when it is required at a level that is beneficial to the productivity of the biomass. This further increases the productivity of biomass using the phototrophic microorganisms.
    In a further preferred embodiment of the invention, removing and / or incorporating CO 2 and / or NH 3 / NH 4 'using the in-situ oxygen formed comprises the step of providing a hydrogen-oxidizing microorganism culture for performing the hydrogenotrophic metabolic pathway.
    Providing the hydrogen-oxidizing microorganism culture, such as bacteria, makes biomass productivity possible by removing and / or absorbing the added CO 2 and / or NH 3 / NH 4 + . This improves the overall operation of the method.
    Preferably, the hydrogen oxidizing microorganisms are at least partially separated from the phototrophic microorganisms. This may entail providing hydrogen-oxidizing microorganisms in a separate compartment or separate reactor from the phototrophic microorganisms. This makes it possible to design the compartments or reactors specifically for the type of microorganisms.
    Preferably, the hydrogen oxidizing microorganisms comprise chemo-autotrophic hydrogen oxidizing bacteria.
    In an advantageous preferred embodiments according to the invention, the phototrophic microorganisms comprise photoautotrophic bacteria.
    Phototrophic microorganisms, in particular phototrophic bacteria, contain photoautotrophic bacteria, use energy, such as light energy, to form carbohydrates using oxygen formed in situ. This is preferably accomplished in a so-called photo bioreactor or other exposed bioreactor, illuminating is preferably accomplished by providing one or more external bed flat panel photobioreactors, external bed tubular photo bioreactor, internal bed fluidized light sources, internal bed illuminated cords of LEDs and / or internal bed quantum dot coated materials such as balls on membranes. These and / or other suitable energy sources make the improvement of biomass productivity possible.
    BE2018 / 5708
    The light spectrum in the reactor is preferably specifically tuned to the absorption properties of the taxa that are cultivated, such as red light for organisms that are mainly dependent on chlorophyll adsorption (eg green (micro) algae), orange light for organisms with a large proportion of phycocyanin in the light-absorbing complex (eg many cyano bacteria), green light for organisms with phycocyanin in their light-absorbing complex (eg, certain cyano bacteria and red algae) or near infrared light for organisms with bacteriochlorophilic absorption maxima in this part of the spectrum (e.g., many photosynthetic bacteria).
    The phototrophic microorganisms in one of the advantageous preferred embodiments according to the invention belong to one or more of the following taxa or phototrophic bacteria:
    purple sulfur bacteria (Chromatiaceae), such as Ectothiorhodospira, Chromatium, Thiocapsa, Thiospirillum, Thiodictyon, and / or Thiopedia;
    - purple non-sulfur bacteria (Ectothiorhodospiraceae, formerly Rhodospirillaceae), such as Rhodomicrobium, Rhodobacter, Rhodopseudomonas, Rhodospirillum, and / or Rhodocycle
    - green sulfur bacteria (Chlorobiaceae), such as Chlorobium, Prostheochloris, Pelodictyon, and / or Chloroherpeton;
    - green non-sulfur bacteria (Chloroflexaceae), such as Chloroflexus;
    heliobacteria, such as Heliobacterium, Helophilum, and / or Heliobacillus;
    - cyanobacteria (Cyanofyta, Cyanophyceae), such as Gloeothece, Synechococcus, Gloeocapsa, Gloeobacter, Dermocapsa, Synechocystis, Plectonema, Xenococcus, Pleurocapsa, Spirulina, Arthrospira, Oscenaum, Penaothyum, Limaothumyl, Limaothumyl, Limaothumyl, Limaothumyl, Limenaoth , Cyanothece, Stigonema, Cyanobotrytis, Westicella, Loriella, and / or Nostichopsis;
    Prochlorophyta, such as Prochlorone and / or Prochlorothrix;
    - Photosynthetic bacteria from other taxa or still unclassified, such as
    Porphyrobacter neustonensis, Roseobacter denitrificans, Erythromicrobium sibericus, Pseudomonas radiora, and / or certain species of Pseudomonas.
    Alternatively or additionally, the phototrophic microorganisms can include one or more of the following eukaryotic microalgae:
    - green algae (Chlorophyceae); such as Chlorella, Chlorococcum, Ettlia, Neochloris, Desmodesmus, Scenedesmus, Selenastrum, Chlamydomonas, Haematococcus, Tetraselmis, and / or Dunaliella;
    Traustochytrids (Traustochytriaceae) such as Aurantiochytrium, Schizochytrium, Thraustochytrium, and / or Ulkenia;
    BE2018 / 5708
    Diatoms (Bacillariophyceae) such as Chaetoceros, Skeletonema, Thallassiosira, Amphora, Navicula, Nitzschia, and / or Phaeodactylum;
    Dinoflagellates (Dinophyceae) such as Cryptothecodinium, Gymnodinium, and / or Oxyrrhis;
    - Euglenophytes (Euglenophyceae) such as Euglena;
    Eustigmatophytes (Eustigmatophyceae) such as Nannochloropsis and / or Ellipsoidon;
    - Raphidophytes (Raphidophyceae) such as Heterosigma and / or Chattonella;
    - Golden algae (Chrysophyceae) such as Dicrateria, Isochrysis, and / or Pavlova; and
    red algae (Rhodophyceae) such as Porphyridium, Galdieria, and / or Cyanidioschyzon.
    It will be clear that combinations of microorganisms can also be used. This contains combinations of species specifically mentioned in this application and also other alternative species, for example hydrogen oxidizing bacteria such as Hydrogenobacter thermophilus, Hydrogenovibrio marinus, and Helicobacter pylori and / or containing non-phototrophic microorganisms such as:
    Ammonium oxidizing and / or denitifying bacteria, such as Nitrosomonas, Nitrosococcus, Nitrobacter, Thiobacillus, Micrococcus, and / or Pseudomonas.
    Anaerobic ammonium oxidizing (Anammox) bacteria, such as Anammoxoglobulus, Brocadia, Jettenia, Kuenenia, and / or Scalindua.
    - Other bacteria, archaea, fungi, protozoa, and / or other (micro) organisms with usable metabolism and / or biomass (products).
    It will be appreciated that combinations of embodiments may also be considered in accordance with the invention. The process can further be applied in a batch reactor or continuous reactor or a combination of a batch reactor and continuous reactor. In the event that the reactor contains a continuous culture, the biomasses are preferably retained by a membrane or in granulate, flakes or biofilm, optionally containing additional materials for immobilisation of the biomass. As mentioned earlier, the different processes mentioned can be carried out in different compartments or in different reactors.
    The present invention is further related to a system for improving biomass productivity, using hydrogenotrophic microorganisms according to the invention, the system comprising:
    - providing a reactor comprising a reactor vessel, at least one gas inlet and / or hydrogen production system, at least one liquid inlet, at least one liquid outlet, and preferably at least one gas outlet, the gas inlet being configured to supply an amount of hydrogen gas;
    BE2018 / 5708
    - at least one hydrogenotrophic microorganism culture for making it possible to carry out the method according to one or more of the preceding claims.
    The system provides the same effects and benefits as described for the method. In particular, the system is capable of performing the method according to one of the embodiments according to the invention.
    Preferably, the system comprises a phototrophic microorganism culture and an in-situ oxygen controller adapted to monitor and / or control the in-situ oxygen production by the phototrophic microorganisms.
    Preferably the reactor vessel comprises hydrogen oxidizing microorganisms.
    In a further advantageous preferred embodiment, the reactor vessel comprises a first vessel compartment for the phototrophic microorganism culture and a second vessel compartment for the hydrogen oxidizing / hydrogenotrophic microorganism culture. This makes a specific design of the individual compartments or vessels possible.
    In a further advantageous embodiment of the invention, the system further comprises a light source.
    The provision of a light source makes it possible to influence or manipulate the reactions and reaction rates that take place in the vessel. The light source may include an external source provided outside the reactor and internal sources provided inside the reactor, including floating sources. Preferably, the system comprises a light source controller operatively connected to the in-situ oxygen controller. This allows the monitoring and / or control of the light source or light sources to control the in-situ oxygen production as determined by the in-situ oxygen controller.
    Further advantages, features and details of the invention are elucidated on the basis of preferred embodiments thereof, wherein reference is made to the accompanying drawings, in which:
    Figure 1 shows an embodiment of a system with gas supplies for improving the biomass growth according to the invention;
    Figures 2 and 3 show an alternative embodiment of a system for improving biomass growth according to the invention with a photobioreactor;
    - Figure 4 A-B shows biomass growth results; and
    - Figure 5 shows some experimental results.
    System 2 (Figure 1) comprises reactor 4 which has a first (hydrogen) inlet 6 and a second (CO 2 ) inlet 8. LEDs 10 are provided around reactor 4 to provide light to reactor 4. It will be clear that light sources can also be provided with alternative embodiments, including floating LEDs inside the reactor. Reactor 4 comprises electronic system 14 with pH electrode 14a, redox electrode 14b and DO electrode 14c. Controller 16 receives and / or
    BE2018 / 5708 sends measurements / control signals 18, 18a, 18b, 18c to the respective electrodes 14. In use, controller 16 sends control signals 20 to LEDs 10 and control sign (s) 22, 26 to control valve 24, 28 which are associated with inlets 6, 8, respectively. Reactor 4 further comprises phototrophic microorganisms 30 and hydrogen oxidizing microorganisms 32.
    Alternative system 102 (Figure 2) includes reactor 104 and gas system 106 for mixing, and optionally humidifying, gas. Gas system 106 produces gas mixture 108 which is provided at inlet 110 of reactor 104. In use, gas sprayer 112 provides gas to reactor 104. In the embodiments shown, transparent flat partition 114 is provided to easily monitor the inside of reactor 104 filled in use with medium and microorganisms. Reactor 104 further comprises a number of openings. For example, apertures 118 can be used for media addition, sampling, measurements, and harvesting. In the embodiments shown, rear partition 120 is provided such that it contains (LED) light sources and optionally an integrated temperature controller. Gas outlet 122 is optionally provided with a capacitor and / or gas analyzer and / or gas recirculation.
    Another alternative system 202 (Figure 3) includes reactor 204 with pipe supports 206 and transparent flow pipes 208 that in use are filled with culture medium and microorganisms. Pipe supports 206 preferably include manifolds. Reactor 204 is provided with inlet 210 and outlets 212 connected to vessel 214 for monitoring and controlling, medium addition, and gas exchange. Biomass harvest opening 216 allows harvesting of biomass that has been produced and provides it to system 218 for sampling, harvesting and further processing. Gas system 120 mixes gases and optionally includes a gas analyzer. Gas system 220 provides a gas mixture at inlet port 222. Controller 224 is operatively coupled to sensors to monitor and control operation.
    It will be clear that various features of the embodiments shown can be exchanged, such as electronic system 18, rear partition 120, vessel 214 and / or one of the other features shown.
    To combat hydrogenotrophic growth mutations, systems 2, 102, 202 preferably include a hydrogen-fed bioreactor and a photo bioreactor, reactors that are optionally integrated, such that the oxidizing equivalents are generated by phototrophs such as microalgae. The phototrophs in reactor 4, 104, 204 can independently use the hydrogen or produce oxygen used by hydrogen oxidizers. According to the advantageous embodiments of the invention, the oxidizing equivalents are produced in the liquid in the reactor. Gas-to-liquid transfer limitations for oxygen are therefore no longer a problem. Because in the preferred embodiments, oxygen is in-situ
    BE2018 / 5708 is generated in reactor 4, 104, 204, the hydrogen is not diluted by air or oxygen additions. Air would introduce an excess of gas that could accumulate in the top of the reactor. The in-situ manufacture of pure oxygen therefore prevents the dilution of hydrogen, and allows a high hydrogen partial pressure, which reduces the gas to liquid transfer limit for hydrogen. Preferably, controller 16 maintains the oxygen partial pressure in reactor 4, 104, 204 in the safe bandwidth by controlling the oxygen production. This is an advantage because the hydrogen supply is often not a suitable control parameter since the hydrogen flow is produced elsewhere by an independent process and therefore cannot easily be reduced or interrupted without the loss of valuable hydrogen. Optionally, the oxygen concentration is kept within the safe bandwidth by reducing the light intensity and / or CO 2 supply for photosynthesis using the oxidation / reduction potential or redox potential, the dissolved oxygen (DO) concentration, and / or the pH, for example.
    Experiments have been performed. In one of the experiments, single-cell microalgae that are commonly used as algae culture (Chlorella sorokiniana) have been used. Results (see Figure 4 A-B) show that under unlimited hydrogen supply, an increase in growth rate of between 24% and 63% compared to growth rates in the control (no extra hydrogen, only light as an energy source) has been observed. In the figures, biomass density is expressed as the natural logarithm of the optical density measured at 750 nm and scaled to an initial value of 1 over time of X in a flat panel photo bioreactor. Slopes of the regression lines represent exponential growth rates. This shows that the effect according to the invention improves the biomass growth and more particularly it shows that "dirty" waste streams can be converted into clean raw material for the cultivation of phototrophs. This allows, for example, full control over the safety of the biomass to be obtained, which can actually be developed without the risk of unwanted (waste water or combustible gases) contaminants.
    Experiments have been conducted to investigate the growth effects of hydrogen supply below limit conditions. In these experiments, microalgae (Chlorella sorokiniana) were cultivated in a photo bioreactor until light-limiting conditions were reached and stable optical densities were measured. Afterwards, hydrogen gas was added to the system in two concentrations, 4 vol% and 8 vol%. The results show that the optical density increased further with an increase in hydrogen concentration. The results (Figure 5) show that the addition of hydrogen under light-limiting conditions results in a higher biomass production.
    BE2018 / 5708
    The present invention is by no means limited to the above described preferred embodiments thereof. The requested rights are determined by the following claims within the scope of which many modifications are conceivable.
    权利要求:
    Claims (15)
    [1]
    A method for increasing biomass productivity using hydrogenotrophic microorganisms, comprising the steps of:
    - providing a reactor comprising a reactor vessel, at least one gas inlet and / or hydrogen production system, at least one fluid inlet, and at least one fluid outlet;
    - providing the reactor with a hydrogenotrophic micro-organism culture;
    - providing a gas supply to the at least one gas inlet, wherein the gas supply comprises an amount of hydrogen and / or producing hydrogen with the hydrogen production system;
    - providing a fluid supply to the at least one fluid inlet;
    - the growth of biomass; and
    - the removal and / or absorption of hydrogen (H 2 ), carbon dioxide (CO 2 ) and / or NH 3 / NH 4 +.
    [2]
    The method of claim 1, further comprising the steps of:
    - providing the reactor with a phototrophic micro-organism culture; and
    - the in-situ formation of oxygen (O 2 ) by the phototrophic microorganisms.
    [3]
    Method according to claim 1 or 2, wherein the method is carried out in a closed system.
    [4]
    A method according to claim 2 or claim 3 so far as dependent on claim 2, further comprising the step of monitoring and / or controlling the in-situ oxygen formed by the phototrophic microorganisms with an in-situ oxygen controller and / or redox controller.
    [5]
    The method of claim 4, wherein the in-situ oxygen controller regulates the in-situ oxygen concentration by controlling the one or more of: light intensity, CO 2 supply, pH redox potential.
    [6]
    A method according to any one of the preceding claims, wherein removing and / or incorporating the H 2 and / or CO 2 and / or NH 3 / NH 4 + using the in-situ formed oxygen comprises the step of providing a hydrogen oxidizing microorganism culture.
    BE2018 / 5708
    [7]
    The method of claim 6, wherein the hydrogen oxidizing microorganisms are at least partially separated from the phototrophic microorganisms.
    [8]
    The method of claim 6 or 7, wherein the hydrogen oxidizing microorganisms comprise chemo-autotrophic nitrogen oxidizing bacteria.
    [9]
    The method of any one of the preceding claims as far as dependent on claim 2, wherein the phototrophic microorganisms comprise photoautotrophic bacteria.
    [10]
    The method of any one of the preceding claims as far as dependent on claim 2, wherein the phototrophic microorganisms comprise eukaryotic microalgae.
    [11]
    A system for improving biomass productivity, using hydrogenotrophic microorganisms, the system comprising:
    - a reactor comprising a reactor vessel, at least one gas inlet and / or hydrogen production system, at least one fluid inlet, at least one fluid outlet, and preferably at least one gas outlet, the gas inlet being configured to supply an amount of hydrogen gas;
    - at least one hydrogenotrophic microorganism culture for making it possible to carry out the method according to one or more of the preceding claims.
    [12]
    The system of claim 11, wherein the reactor vessel comprises a phototrophic microorganism culture and an in-situ oxygen controller configured to monitor and / or control the in-situ oxygen production by the phototrophic microorganisms.
    [13]
    The system of claim 12, wherein the reactor vessel comprising a first reactor compartment for the phototrophic microorganism culture and a second reactor compartment for the hydrogenotrophic microorganism culture.
    [14]
    The system of claim 12 or 13, further comprising a light source.
    [15]
    The system of claim 14, further comprising a light source controller operatively connected to the in-situ oxygen controller.
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    同族专利:
    公开号 | 公开日
    NL2019733B1|2019-04-23|
    EP3697891A1|2020-08-26|
    WO2019078713A1|2019-04-25|
    BE1025911A1|2019-08-07|
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    法律状态:
    2020-01-29| FG| Patent granted|Effective date: 20191224 |
    优先权:
    申请号 | 申请日 | 专利标题
    NL2019733A|NL2019733B1|2017-10-16|2017-10-16|Method and system for the enhancement of productivity of hydrogenotrophic micro-organisms|
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